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programme SharCo

LogoSharco_arbreVert(w SharCo

Consiglio Nazionale delle Ricerche-Istituto di Virologia Vegetale - Italy

Specifically involved in basic and applied research on viruses and virus diseases affecting Mediterranean crops

The Institute of Plant Virology (IVV) belongs to the National Research Council (CNR), a public research organization. The operative unit of Bari (IVV-BA) is specifically involved in basic and applied research on viruses and virus diseases affecting Mediterranean crops. Activities on PPV included eradication programs, development of diagnostic tools, strain characterization, establishement of guidelines and protocols for the production and propagation of certified plant materials. The IVV-BA has a long established cooperation with the Department of Plant Protection and Applied Microbiology (DPPMA) of the University of Bari and the Centro di Studio e Sperimentazione in Agricoltura “Basile Caramia” (CRSA), Locorotondo (Bari).

Key persons involved

  • Dr Donato BOSCIA (CNR-IVV)
    Plant virologist specialised in serology (development and application of polyclonal and monoclonal antibodies applied to strain identification.

  • Prof Vito SAVINO (U. of Bari)
    Plant virologist, whose main field is production of virus-free material. He is advisor for certification schemes of the Phytosanitary Authority.

  • Dr Angelantonio MINAFRA (CNR-IVV)
    Plant virologist specialised in molecular detection of viruses of grapevine and stone fruit. Main field is the diagnosis of phloem-limited filamentous viruses by RT-PCR and hybridization.

  • Dr Antonio CARDONE (Technical Director at CRSA)
    15-years experience in plant protection, experimental field management and sanitary selection, in vitro propagation and sanitation.

Publications

  • Bazzoni A., Amenduni T., Minafra A., Oukaci G., Boscia D., Savino V. 2005. Development and application of a protocol for the evaluation of resistance to PPV in apricot. Phytopathologia Polonica 36, 47-52.

  • Myrta A., Al Rwahnih M., Savino V. 2005. Presence of a recombinant isolate of Plum pox virus in Apulia. Journal of Plant Pathology 87, 127-130.

  • Myrta A., Boscia D., Potere O., Kolber M., Nemeth M., Di Terlizzi B., Cambra M., Savino V. 2001. Existence of two serological subclusters of Plum pox virus, strain M. European Journal of Plant Pathology 107, 845-848.

  • Boscia D., Zeramdini H., Cambra M., Potere O., Gorris M.T., Myrta A., Di Terlizzi B., Savino V. 1997. Production and characterization of a monoclonal antibody specific to the serotype M of plum pox potyvirus. European Journal of Plant Pathology, 103, 477-480.

Material resources

A screenhouse at DPPMA fully dedicated to PPV-infected Prunus spp. Two screenhouses at CRSA are devoted to testing and challenging for PPV resistance on cultivars and breeding lines. Electron microscope: a “Philips Morgagni” transmission electron microscope will be used for all the potential needs in the project (immunoelectro-microscopy; thin section analysis and cytopathology, etc.). Monoclonal antibody laboratory: fully equipped for MAbs production and analysis. Laboratory for in vitro culture: with annexed climatized growth-chamber and heat therapy room, equipped for micropropagation and sanitation by meristem culture. Laboratory for molecular detection: equipped with a real-time PCR apparatus, hybridization ovens, radioisotopes facility. DPPMA also owns glasshouse boxes authorized for maintenance of class 2 GMOs (plants and microorganisms).