The Consejo Superior de Investigaciones Científicas (CSIC), Spain is the largest public research organization of Spain and it is distinguished by its multidisciplinary nature, approaching all knowledge areas, from basic research to top-most advanced technological developments.
The CSIC is responsible for 20% of international scientific publications of Spain and 30% of the Spanish European patents. The Centro Nacional de Biotecnología (CNB) is one of the 116 institutes of the CSIC. It was opened in 1992, and is organized in five Departments: Molecular and Cell Biology, Macromolecule Structure, Immunology and Oncology, Microbial Biotechnology and Plant Molecular Genetics, and several technical Units, that are usually linked to research groups of the Departments. The aims of the CNB are to develop basic and applied research programs, to train young scientist, and to disseminate the technologies developed among other research centers and industry. The CNB is willing to contribute its research infrastructure for the successful completion of the project.
Key persons involved
Prof. Juan Antonio García
Working in the molecular biology of Plum pox virus from 1984.
Dr. Carmen Simón-Mateo
8 years experience in the molecular biology of Plum pox virus
Senior technician with more than 20 years experience in molecular biology, 13 of them working with plant molecular genetics.
A Postdoctoral Scientist -to be hired-
Experience in molecular biology and plant pathology
Two other postdoctoral scientists and four other predoctoral researchers. All of them will collaborate in the development of the project.
D. Chen, S. Juárez, L. Hartweck, J.M. Alamillo, C. Simón-Mateo, J.J. Pérez, M.R. Fernández-Fernández, N.E. Olszewski and J.A. García (2005). Identification of SECRET AGENT as the O-GlcNAc transferase that participates in Pum pox virus infection. J. Virol. 79, 9381-9387.
J.M. Alamillo, P. Sáenz, and J.A. García (2006). Salicylic acid-mediated and RNA silencing defense mechanisms cooperate in the restriction of Plum pox virus systemic spread in tobacco. Plant J. 48, 217-227.
C. Simón-Mateo and J.A. García (2006). Mi-RNA-guided processing impairs Plum pox virus, replication but the virus readily evolves to escape this silencing mechanism. J. Virol. 80, 2429-2436.
A. Valli, A.M. Martín-Hernández, J.J. López-Moya and J.A. García (2006). RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus (CVYV), a member of the family Potyviridae that lacks the cysteine protease HCPro. J. Virol. 80, 10055-10063.
J.A. García and C. Simón-Mateo (2006). A micropunch against plant viruses. Nat. Biotechnol. 24, 1358-1359.
The CNB possesses all the equipment required for research in plant molecular biology and virology. In addition, the CNB has centralized technical Units that provide support to the research of the different laboratories. I would like to remark the availability of greenhouses and climate rooms with appropriate biosafety levels, and Plant Cell Culture, Plant Transformation, Genomics and Proteomics services that will be used in the development of the project.