IVIA is a public research institution dedicated to agricultural sciences. The Institution is organized in seven Centres. In the SharCo project two Centres are involved: Plant Protection and Biotechnology (PPB) and Fruit Production (FP). The Department of Virology and Immunology from PPB Centre is the Reference Laboratory of the Spanish Ministry of Agriculture for woody plant viruses. It has 30 years experience in research on detection and characterisation of plant viruses of woody species. The Department of Fruit Breeding from the FP Centre is in charge of breeding stone fruits resistant to PPV and has expertise in molecular genetics. Both Centres are leading the applied research and management of sharka in Spain and have participated in international projects, expert-groups, co-operation and consultancies related to this topic.
Key persons involved
Centre of Plant Protection and Biotechnology Virology and Immunology (IVIA partner P6a)
• Prof. Mariano Cambra, research plant virologist and epidemiologist from 1977, expert in the design of serological and molecular diagnostic methods.
• Dr. Antonio Olmos, research plant biologist expert in molecular methods.
• Dr. Aranzazu Moreno, research plant biologist expert in epidemiology.
Centre of Fruit Production. Fruit Breeding (IVIA partner P6b)
• Dr. Maria L. Badenes, research scientist expert in genetics and breeding of fruit crops. • Prof. Gerardo Llácer, expert on graft-transmissible fruit trees diseases.
• Dr. Carlos Romero, research scientist in charge of the quarantine and certification.
Cambra, M., Capote, N., Cambra, M.A, Llacer, G., Botella, P., Lopez-Quilez, A. (2006). Epidemiology of sharka disease in Spain. EPPO Bulletin 36, 271-275.
Olmos, A., Bertolini, E., Gil, M. Cambra,M. (2005). Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids. J. Virol. Methods 128, 151-155.
Capote, N. Gorris, MT., Martínez, MC., Asensio, M., Olmos, A., Cambra, M. (2006). Interference between D and M types of Plum pox virus in Japanese plums assessed by specific monoclonal antibodies and quantitative real-time RT-PCR. Phytopathology 96, 320-325.
Vilanova, S., Romero, C., Abbott, A.G., Llácer, G., Badenes, M.L. (2003). An apricot (Prunus armeniaca L.) F2 progeny linkage map based on SSR and AFLP markers, mapping plum pox virus resistance and self-incompatibility traits. Theor. Appl.Genetics, 107: 239-247.
Vilanova, S., Soriano, JM., Lalli, DA., Romero, C., Abbott, AG., Llácer, G., Badenes, ML. (2006). Development of microsatellite markers located in the Linkage Group one of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library. Molecular Ecology Notes 6:789-791.
The IVIA Institution have all facilities to carry out the project, including insect-proof tunnels and greenhouses, bio-security laboratories P2 and P3 levels, laboratories of virology, breeding and molecular biology, and 62 ha experimental plots. In addition, the IVIA have a fruit stone experimental net which includes six Experimental Stations with technical personnel, Prunus nurseries and fruit tree collections available.