En naviguant sur notre site vous acceptez l'installation et l'utilisation des cookies sur votre ordinateur. En savoir +

Menu Logo Principal FP7

programme SharCo

LogoSharco_arbreVert(w SharCo

Simple and efficient biolistic procedure for the plant transfection with cDNA clones of RNA viruses

Acta Virologica

L. Predajňa, A. Nagyová , Z. Šubr *
*Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovak Republic


Simple, fast, low-cost, and efficient procedure for DNA delivery to the cell nuclei of whole plants was developed. The procedure was optimized for the Plum pox virus (PPV) and its host Nicotiana benthamiana. It is based on the leaf bombardment with tungsten microparticles with bound DNA using common air gun. The procedure did not require special equipment and source of driving gas. The transfection efficiency obtained by the newly developed procedure was close to 100%, but this level dropped with the age of bombarded plants.

The article can be consulted here.